Date Published 
May 2013

Page Count 
106

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 Digital PCR Article in Biocompare 

  

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Digital PCR Technology Report: An Insight to Vendors, Costs & the End User Community - Tables and Figures

Tables: 

Table 3.1: Digital PCR (dPCR) vs. Quantitative real-time PCR (qPCR)
Table 5.1: Summary of RainDrop™ Digital PCR System
Table 6.1: Summary of QX100™ Droplet Digital™ PCR System
Table 7.1: Summary of QuantStudio 3D™ Digital PCR System
Table 8.1: Summary of BioMark HD™ System, EP1™ System, 12.765 Digital Array™ IFC, and qdPCR 37K™ IFC
Table 9.1: Comparison of Specifications

Figures: 

Figure 1.1: Flowchart of real-time PCR process
Figure 1.2: Depiction of digital PCR process
Figure 5.1: RainDrop Source: Left. RainDrop Sense: Right
Figure 5.2: RainDrop chip. Up to 8 samples can be loaded
Figure 5.3: Adjusting the concentration of the probes causes them to shift and display different intensities
Figure 5.4: Workflow of RainDrop Digital PCR System
Figure 6.1: QX100 Droplet Digital PCR System components
Figure 6.2: Microfluidics combines oil (North and South channels) with the sample (West channel) to create nanoliter droplets (East channel)
Figure 6.3: Schematic for detecting multiple DNA sequences within a droplet digital PCR fluorescence amplitude scatter plot
Figures 6.4: Positive sequences labeled with FAM (blue), FAM+HEX (green), and HEX (black) dyes
Figure 6.5: Positive sequences labeled with FAM or FAM+HEX (brown), FAM or HEX (blue), and, FAM+HEX or HEX (green) dyes
Figure 6.6: Positive sequences labeled with FAM, FAM+HEX, or HEX dyes
Figure 6.7: Workflow of QX100 Droplet Digital PCR System
Figure 6.8: Step 1: Prepare samples
Figure 6.9: Step 2: Generate droplets using the QX100 droplet generator
Figure 6.10: Step 3: Thermal cycle samples
Figure 6.11: Step 4: Load samples onto the QX100 droplet reader
Figure 6.12: Step 5: Analyze results
Figure 6.13: Step 6: Visualize data
Figure 7.1: QuantStudio 3D Digital PCR System
Figure 7.2: The chip can hold one sample at a time, partitioning it across 20,000 wells for analysis
Figure 7.3: Workflow of QuantStudio 3D Digital PCR System
Figure 8.1: BioMark HD System
Figure 8.2: EP1 System
Figure 8.3: qdPCR 37K IFC
Figure 8.4: Real-time detection captures false positive using the BioMark HD System
Figures 8.5-8.9: Workflow of BioMark HD System