Length 164 pages

Date published June 2009

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Next-Generation Sequencing: Solving the Genome Table of Contents

 

Author: Ken Rubenstein, PhD

Chapter 1
INTRODUCTION
1.1. Background
1.2. Scope and Nature of the Report

Chapter 2 
HISTORY AND EVOLUTION OF SEQUENCING TECHNOLOGY
2.1. Frederick Sanger & Early DNA Sequencing
2.2. Leroy Hood Pioneers Semi-Automated Sequencing
2.3. The Human Genome Project and Celera Genomics: Race On
2.4. The $1,000 Genome & Next-Generation Systems

Chapter 3 
SEQUENCING TECHNOLOGIES: NEXT-GENERATION AND BEYOND
3.1. Current Next-Generation Sequencers
Roche/454 Life Sciences
Illumina
Applied Biosystems
Helicos
3.2. Sequencers Currently in Development
Complete Genomics
BioNanomatrix
VisiGen
Pacific Biosciences
Dover Systems
Intelligent Bio-Systems
ZS Genetics
Reveo
LightSpeed Genomics
3.3. Nanopore-Based Systems
NABsys
Oxford Nanopore Technologies
3.4. Performance Comparisons
3.5. Bioinformatics
3.6. Sample Preparation
3.7. National Human Genome Research Institute (NHGRI) Funding
3.8. Intellectual Property Issues

Chapter 4
APPLICATIONS OF NEXT-GENERATION SEQUENCING TECHNOLOGIES
4.1. Whole-Genome Sequencing
The 1,000 Genomes Project
Exon Sequencing
Accuracy and Precision in Genetic Variation Studies
4.2. RNA Sequencing
4.3. Gene Regulation and Control Analysis
4.4. Disease Diagnosis and Prediction
Cancer Genomics
Personal Genomics
4.5. Metagenomics

Chapter 5
MARKET DYNAMICS
5.1. Deals
5.2. User Survey Results

Chief Application for Next-Generation DNA Sequencing
Main Organism for Next-Generation Sequencing
User Experience with Next-Generation Platform, Industry Segment
Next-Generation Platform Predicted to Dominate in 2–3 Years
Most Exciting Third-Generation Platform
Applications to Benefit Most from Next-Generation Sequencing
Research Areas to Benefit Most from Next-Generation Sequencing
Greatest Technical Concern about Next-Generation Sequencing Platforms
Greatest Computational/IT Concern
Adequate Cost/Benefit from Next-Generation Sequencing
Cost of Full Human Genome Sequence by 2010
Opinion on Platform Market Dominance
Future of Sanger Sequencing
Most Important Factors in Selecting a Next-Generation System
Opinion on Acquiring Sequence from a Service Provider
5.3. The Competitive Environment

Chapter 6
OBSERVATIONS AND CONCLUSIONS

Chapter 7
INTERVIEW TRANSCRIPTS
7.1. Clifford Reid, PhD, Chairman, President, & CEO, Complete Genomics
7.2. Patrice Milos, PhD, VP & Chief Scientific Officer, Helicos BioSciences
7.3. Steven Salzberg, PhD, Director, Center for Bioinformatics and Computational Biology, University of Maryland
7.4. Todd Smith, PhD, Chief Science Officer, Geospiza
7.5. Anonymous Academic Researcher Active in Next-Generation Sequencing
7.6. Stephen Naylor, PhD, CSO & Chairman, Predictive Physiology and Medicine (PPM)

References

Company Index with Web Addresses