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NGS Leaders 

Date published June 2011

 

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Next Generation Sequencing Generates Momentum: Markets Respond to Technology and Innovation Advances - Tables and Figures

 

Author: Ken Rubenstein, PhD

FIGURES
Figure 2.1  Sanger Sequencing Methodology 4
Figure 3.1  Principles and workflow for Illumina sequencing 11
Figure 3.2  The SOLiD sequencing by ligation principle 13
Figure 3.3  Roche/454 original sample preparation and sequencing scheme 18
Figure 3.4  Pacific Biosciences’ single-molecule sequencing principle 20
Figure 3.5  Life Technologies’ SOLiD system software support 28
Figure 3.6  Principle of the Agilent SureSelectTarget Enrichment system 33
Figure 3.7  The RainDance Technologies RDT 1000 sample enrichment system 34
Figure 3.8  Overview of Nextera fragmentation and tagging technology 35
Figure 4.1  Oxford Nanopore exonuclease sequencing principle 38
Figure 4.2  The principle underlying NobleGen’s sequencing technology 50
Figure 5.1  Type of Institution 63
Figure 5.2  Type of sequencing facility 64
Figure 5.3  Position in the organization 64
Figure 5.4  Informatics sources 66
Figure 5.5  Low-cost sequencer acquisition 67
Figure 5.6  Low-cost sequence selection 67
Figure 5.7.  Pacific Biosciences system plans 68
Figure 5.8.  Future interest in single-molecule sequencing 68
Figure 5.9.  Nanopore Sequencer Timing 68
Figure 5.10.  70
Figure 5.11.  71
Figure 5.12.  71
Figure 5.13. 72
Figure 5.14. 72

TABLES
Table 3.1  Performance comparison estimates for systems currently on the market or launched as of October 2010(for fragment, not mate-paired reads) 26
Table 5.1  NGS Deals Relating to Sequencing Instrumentation 59
Table 5.2  NGS Deals Relating to Informatics 60
Table 5.3  NGS Deals Relating to Sample Preparation 62
Table 5.4  In-house vs outsource 63
Table 5.5  Sequencing focus 65
Table 5.6  Organisms sequenced 65
Table 5.7  Application areas 65
Table 5.8.  Sequencer attribute rankings 66
Table 5.9.  Desired nanopore system attributes 69